Isolation of detergent resistant microdomains from cultured neurons: detergent dependent alterations in protein composition.

ABSTRACT:

Background

Membrane rafts are small highly dynamic sterol- and sphingolipid-enriched membrane domains that have received considerable attention due to their role in diverse cellular functions. More recently the involvement of membrane rafts in neuronal processes has been highlighted since these specialized membrane domains have been shown to be involved in synapse formation, neuronal polarity and neurodegeneration. Detergent resistance followed by gradient centrifugation is often used as first step in screening putative membrane raft components. Traditional methods of raft isolation employed the nonionic detergent Triton X100. However successful separation of raft from non-raft domains in cells is dependent on matching the detergent used for raft isolation to the specific tissue un

SUBMITTER: Williamson R 

PROVIDER: S-EPMC2955047 | biostudies-literature | 2010 Sep

REPOSITORIES: biostudies-literature