Detecting variants with Metabolic Design, a new software tool to design probes for explorative functional DNA microarray development.

ABSTRACT:

Background

Microorganisms display vast diversity, and each one has its own set of genes, cell components and metabolic reactions. To assess their huge unexploited metabolic potential in different ecosystems, we need high throughput tools, such as functional microarrays, that allow the simultaneous analysis of thousands of genes. However, most classical functional microarrays use specific probes that monitor only known sequences, and so fail to cover the full microbial gene diversity present in complex environments. We have thus developed an algorithm, implemented in the user-friendly program Metabolic Design, to design efficient explorative probes.

Results

First we have validated our approach by studying eight enzymes involved in the degradation of polycyclic aromatic hydroc

SUBMITTER: Terrat S 

PROVIDER: S-EPMC2955052 | biostudies-literature | 2010 Sep

REPOSITORIES: biostudies-literature