Label-free cellular imaging by broadband coherent anti-Stokes Raman scattering microscopy.

ABSTRACT: Raman microspectroscopy can provide the chemical contrast needed to characterize the complex intracellular environment and macromolecular organization in cells without exogenous labels. It has shown a remarkable ability to detect chemical changes underlying cell differentiation and pathology-related chemical changes in tissues but has not been widely adopted for imaging, largely due to low signal levels. Broadband coherent anti-Stokes Raman scattering (B-CARS) offers the same inherent chemical contrast as spontaneous Raman but with increased acquisition rates. To date, however, only spectrally resolved signals from the strong CH-related vibrations have been used for CARS imaging. Here, we obtain Raman spectral images of single cells with a spectral range of 600-3200 cm⁻¹, including signatu

SUBMITTER: Parekh SH 

PROVIDER: S-EPMC2955349 | biostudies-literature | 2010 Oct

REPOSITORIES: biostudies-literature