DNA damage responses in human induced pluripotent stem cells and embryonic stem cells.

ABSTRACT:

Background

Induced pluripotent stem (iPS) cells have the capability to undergo self-renewal and differentiation into all somatic cell types. Since they can be produced through somatic cell reprogramming, which uses a defined set of transcription factors, iPS cells represent important sources of patient-specific cells for clinical applications. However, before these cells can be used in therapeutic designs, it is essential to understand their genetic stability.

Methodology/principal findings

Here, we describe DNA damage responses in human iPS cells. We observe hypersensitivity to DNA damaging agents resulting in rapid induction of apoptosis after γ-irradiation. Expression of pluripotency factors does not appear to be diminished after irradiation in iPS cells. Following irradi

SUBMITTER: Momcilovic O 

PROVIDER: S-EPMC2955528 | biostudies-literature | 2010 Oct

REPOSITORIES: biostudies-literature