The lack of ADAM17 activity during embryonic development causes hemorrhage and impairs vessel formation.

ABSTRACT:

Background

ADAM17/TACE activity is important during embryonic development. We wished to investigate possible roles of this metalloprotease, focusing on vascular development.

Methodology/principal findings

Mice mutant in the enzymatic activity of ADAM17 were examined at various stages of embryonic development for vascular pattern and integrity using markers for vessel wall cells. We observed hemorrhage and edema starting at embryonic day E14.5 and becoming more severe as development proceeded; prior to embryonic day E14.5, embryos appeared normal. Staining for PECAM-1/CD31 revealed abnormalities in the patterns of branching of the embryonic vasculature at E14.5.

Conclusions/significance

These abnormalities preceded association of pericytes or monocyte/macrophage cells

SUBMITTER: Canault M 

PROVIDER: S-EPMC2955552 | biostudies-literature | 2010 Oct

REPOSITORIES: biostudies-literature