Multiplexed methylation profiles of tumor suppressor genes and clinical outcome in lung cancer.

ABSTRACT:

Background

Changes in DNA methylation of crucial cancer genes including tumor suppressors can occur early in carcinogenesis, being potentially important early indicators of cancer. The objective of this study was to examine a multiplexed approach to assess the methylation of tumor suppressor genes as tumor stratification and clinical outcome prognostic biomarkers for lung cancer.

Methods

A multicandidate probe panel interrogated DNA for aberrant methylation status in 18 tumor suppressor genes in lung cancer using a methylation-specific multiplex ligation-dependent probe amplification assay (MS-MLPA). Lung cancer cell lines (n = 7), and primary lung tumors (n = 54) were examined using MS-MLPA.

Results

Genes frequently methylated in lung cancer cell lines including SCGB

SUBMITTER: Castro M 

PROVIDER: S-EPMC2955578 | biostudies-literature | 2010 Sep

REPOSITORIES: biostudies-literature