Neisseria meningitidis rifampicin resistant strains: analysis of protein differentially expressed.

ABSTRACT:

Background

Several mutations have been described as responsible for rifampicin resistance in Neisseria meningitidis. However, the intriguing question on why these strains are so rare remains open. The aim of this study was to investigate the protein content and to identify differential expression in specific proteins in two rifampicin resistant and one susceptible meningococci using two-dimensional electrophoresis (2-DE) combined with mass spectrometry.

Results

In our experimental conditions, able to resolve soluble proteins with an isoelectric point between 4 and 7, twenty-three proteins have been found differentially expressed in the two resistant strains compared to the susceptible. Some of them, involved in the main metabolic pathways, showed an increased expression, mai

SUBMITTER: Neri A 

PROVIDER: S-EPMC2955632 | biostudies-literature | 2010 Sep

REPOSITORIES: biostudies-literature