Thaumatin-like proteins are differentially expressed and localized in phloem tissues of hybrid poplar.

ABSTRACT:

Background

Two thaumatin-like proteins (TLPs) were previously identified in phloem exudate of hybrid poplar (Populus trichocarpa x P. deltoides) using proteomics methods, and their sieve element localization confirmed by immunofluorescence. In the current study, we analyzed different tissues to further understand TLP expression and localization in poplar, and used immunogold labelling to determine intracellular localization.

Results

Immunofluorescence using a TLP antiserum confirmed the presence of TLP in punctate, organelle-like structures within sieve elements. On western blots, the antiserum labeled two constitutively expressed proteins with distinct expression patterns. Immunogold labelling suggested that TLPs are associated with starch granules and starch-containing pla

SUBMITTER: Dafoe NJ 

PROVIDER: S-EPMC2956541 | biostudies-literature | 2010 Aug

REPOSITORIES: biostudies-literature