Whole genome sequencing of enriched chloroplast DNA using the Illumina GAII platform.

ABSTRACT:

Background

Complete chloroplast genome sequences provide a valuable source of molecular markers for studies in molecular ecology and evolution of plants. To obtain complete genome sequences, recent studies have made use of the polymerase chain reaction to amplify overlapping fragments from conserved gene loci. However, this approach is time consuming and can be more difficult to implement where gene organisation differs among plants. An alternative approach is to first isolate chloroplasts and then use the capacity of high-throughput sequencing to obtain complete genome sequences. We report our findings from studies of the latter approach, which used a simple chloroplast isolation procedure, multiply-primed rolling circle amplification of chloroplast DNA, Illumina Genome Analyzer I

SUBMITTER: Atherton RA 

PROVIDER: S-EPMC2956718 | biostudies-literature | 2010 Sep

REPOSITORIES: biostudies-literature