Detection of sexually transmitted infection and human papillomavirus in negative cytology by multiplex-PCR.

ABSTRACT:

Background

The aim of this study was to determine the prevalence of human papillomavirus (HPV) and 15 species that cause sexually transmitted infections (STIs) in negative cytology. In addition, we compared the diagnostic performance of multiplex polymerase chain reaction (PCR) with widely available techniques used to detect HPV.

Methods

We recruited 235 women of reproductive age who had negative cytology findings in a liquid-based cervical smear. STIs were identified by multiplex PCR, and HPV genotypes by multiplex PCR, hybrid capture 2, and DNA microaray; discordant results were analyzed by direct sequencing.

Results

Approximately 96.6% of patients with negative cytology results were positive for pathogens that cause STIs. The pathogens most frequently detected were

SUBMITTER: Shim HS 

PROVIDER: S-EPMC2956726 | biostudies-literature | 2010 Sep

REPOSITORIES: biostudies-literature