Oct-3/4 regulates stem cell identity and cell fate decisions by modulating Wnt/β-catenin signalling.

ABSTRACT: Although the transcriptional regulatory events triggered by Oct-3/4 are well documented, understanding the proteomic networks that mediate the diverse functions of this POU domain homeobox protein remains a major challenge. Here, we present genetic and biochemical studies that suggest an unexpected novel strategy for Oct-3/4-dependent regulation of embryogenesis and cell lineage determination. Our data suggest that Oct-3/4 specifically interacts with nuclear β-catenin and facilitates its proteasomal degradation, resulting in the maintenance of an undifferentiated, early embryonic phenotype both in Xenopus embryos and embryonic stem (ES) cells. Our data also show that Oct-3/4-mediated control of β-catenin stability has an important function in regulating ES cell motility. Down-regulation of

SUBMITTER: Abu-Remaileh M 

PROVIDER: S-EPMC2957205 | biostudies-literature | 2010 Oct

REPOSITORIES: biostudies-literature