Proteomic analysis of Salmonella enterica serovar Enteritidis following propionate adaptation.

ABSTRACT:

Background

Salmonella Enteritidis is a highly prevalent and persistent foodborne pathogen and is therefore a leading cause of nontyphoidal gastrointestinal disease worldwide. A variety of stresses are endured throughout its infection cycle, including high concentrations of propionate (PA) within food processing systems and within the gut of infected hosts. Prolonged PA exposure experienced in such milieus may have a drastic effect on the proteome of Salmonella Enteritidis subjected to this stress.

Results

In this study, we used 2 D gel electrophoresis to examine the proteomes of PA adapted and unadapted S. Enteritidis and have identified five proteins that are upregulated in PA adapted cultures using standard peptide mass fingerprinting by MALDI-TOF-MS and sequencing by MALD

SUBMITTER: Calhoun LN 

PROVIDER: S-EPMC2957393 | biostudies-literature | 2010 Sep

REPOSITORIES: biostudies-literature