Characterizing tyrosine phosphorylation signaling in lung cancer using SH2 profiling.

ABSTRACT:

Background

Tyrosine kinases drive the proliferation and survival of many human cancers. Thus profiling the global state of tyrosine phosphorylation of a tumor is likely to provide a wealth of information that can be used to classify tumors for prognosis and prediction. However, the comprehensive analysis of tyrosine phosphorylation of large numbers of human cancer specimens is technically challenging using current methods.

Methodology/principal findings

We used a phosphoproteomic method termed SH2 profiling to characterize the global state of phosphotyrosine (pTyr) signaling in human lung cancer cell lines. This method quantifies the phosphorylated binding sites for SH2 domains, which are used by cells to respond to changes in pTyr during signaling. Cells could be grouped ba

SUBMITTER: Machida K 

PROVIDER: S-EPMC2957407 | biostudies-literature | 2010 Oct

REPOSITORIES: biostudies-literature