DiScRIBinATE: a rapid method for accurate taxonomic classification of metagenomic sequences.

ABSTRACT:

Background

In metagenomic sequence data, majority of sequences/reads originate from new or partially characterized genomes, the corresponding sequences of which are absent in existing reference databases. Since taxonomic assignment of reads is based on their similarity to sequences from known organisms, the presence of reads originating from new organisms poses a major challenge to taxonomic binning methods. The recently published SOrt-ITEMS algorithm uses an elaborate work-flow to assign reads originating from hitherto unknown genomes with significant accuracy and specificity. Nevertheless, a significant proportion of reads still get misclassified. Besides, the use of an alignment-based orthology step (for improving the specificity of assignments) increases the total binning time

SUBMITTER: Ghosh TS 

PROVIDER: S-EPMC2957682 | biostudies-literature | 2010 Oct

REPOSITORIES: biostudies-literature