Metabolic pathway reconstruction of eugenol to vanillin bioconversion in Aspergillus niger.

ABSTRACT: Identification of missing genes or proteins participating in the metabolic pathways as enzymes are of great interest. One such class of pathway is involved in the eugenol to vanillin bioconversion. Our goal is to develop an integral approach for identifying the topology of a reference or known pathway in other organism. We successfully identify the missing enzymes and then reconstruct the vanillin biosynthetic pathway in Aspergillus niger. The procedure combines enzyme sequence similarity searched through BLAST homology search and orthologs detection through COG & KEGG databases. Conservation of protein domains and motifs was searched through CDD, PFAM & PROSITE databases. Predictions regarding how proteins act in pathway were validated experimentally and also compared with reported data.

SUBMITTER: Srivastava S 

PROVIDER: S-EPMC2957766 | biostudies-literature | 2010 Jan

REPOSITORIES: biostudies-literature