Sequence and spatial expression of zebrafish (Danio rerio) alphaA-crystallin.

ABSTRACT:

Purpose

To determine the nucleotide sequence, amino acid sequence and tissue specificity of zebrafish alphaA-crystallin.

Methods

RACE, both 3' and 5', was used to clone the zebrafish alphaA-crystallin gene. The peptide sequence of the encoded protein was deduced and compared to cavefish, shark, amphibian, bird and human orthologues using the CLUSTAL W algorithm. alphaA-crystallin transcript was evaluated in brain, heart, lens, liver, skeletal muscle/skin, and spleen by semi-quantitative RT-PCR.

Results

The 173 amino acid sequence of zebrafish alphaA-crystallin was determined to be 73% and 86% similar to its human and cavefish orthologues, respectively. We detected high expression of zebrafish alphaA-crystallin in the lens and very low expression in liver and spleen.

SUBMITTER: Runkle S 

PROVIDER: S-EPMC2957823 | biostudies-literature | 2002 Mar

REPOSITORIES: biostudies-literature