Disruption of gap junctions attenuates aminoglycoside-elicited renal tubular cell injury.

ABSTRACT:

Background and purpose

Gap junctions play important roles in the regulation of cell phenotype and in determining cell survival after various insults. Here, we investigated the role of gap junctions in aminoglycoside-induced injury to renal tubular cells.

Experimental approach

Two tubular epithelial cell lines NRK-E52 and LLC-PK1 were compared for gap junction protein expression and function by immunofluorescent staining, Western blot and dye transfer assay. Cell viability after exposure to aminoglycosides was evaluated by WST assay. Gap junctions were modulated by transfection of the gap junction protein, connexin 43 (Cx43), use of Cx43 siRNA and gap junction inhibitors.

Key results

NRK-E52 cells expressed abundant Cx43 and were functionally coupled by gap junctional

SUBMITTER: Yao J 

PROVIDER: S-EPMC2958649 | biostudies-literature | 2010 Aug

REPOSITORIES: biostudies-literature