SIRT1 undergoes alternative splicing in a novel auto-regulatory loop with p53.

ABSTRACT:

Background

The NAD-dependent deacetylase SIRT1 is a nutrient-sensitive coordinator of stress-tolerance, multiple homeostatic processes and healthspan, while p53 is a stress-responsive transcription factor and our paramount tumour suppressor. Thus, SIRT1-mediated inhibition of p53 has been identified as a key node in the common biology of cancer, metabolism, development and ageing. However, precisely how SIRT1 integrates such diverse processes remains to be elucidated.

Methodology/principal findings

Here we report that SIRT1 is alternatively spliced in mammals, generating a novel SIRT1 isoform: SIRT1-ΔExon8. We show that SIRT1-ΔExon8 is expressed widely throughout normal human and mouse tissues, suggesting evolutionary conservation and critical function. Further studies demon

SUBMITTER: Lynch CJ 

PROVIDER: S-EPMC2958826 | biostudies-literature | 2010 Oct

REPOSITORIES: biostudies-literature