Scoring function to predict solubility mutagenesis.

ABSTRACT:

Background

Mutagenesis is commonly used to engineer proteins with desirable properties not present in the wild type (WT) protein, such as increased or decreased stability, reactivity, or solubility. Experimentalists often have to choose a small subset of mutations from a large number of candidates to obtain the desired change, and computational techniques are invaluable to make the choices. While several such methods have been proposed to predict stability and reactivity mutagenesis, solubility has not received much attention.

Results

We use concepts from computational geometry to define a three body scoring function that predicts the change in protein solubility due to mutations. The scoring function captures both sequence and structure information. By exploring the literat

SUBMITTER: Tian Y 

PROVIDER: S-EPMC2958853 | biostudies-literature | 2010 Oct

REPOSITORIES: biostudies-literature