Identification and properties of the crenarchaeal single-stranded DNA binding protein from Sulfolobus solfataricus.

ABSTRACT: Single-stranded DNA binding proteins (SSBs) play central roles in cellular and viral processes involving the generation of single-stranded DNA. These include DNA replication, homologous recombination and DNA repair pathways. SSBs bind DNA using four 'OB-fold' (oligonucleotide/oligosaccharide binding fold) domains that can be organised in a variety of overall quaternary structures. Thus eubacterial SSBs are homotetrameric whilst the eucaryal RPA protein is a heterotrimer and euryarchaeal proteins vary significantly in their subunit compositions. We demonstrate that the crenarchaeal SSB protein is an abundant protein with a unique structural organisation, existing as a monomer in solution and multimerising on DNA binding. The protein binds single-stranded DNA distributively with a binding si

SUBMITTER: Wadsworth RI 

PROVIDER: S-EPMC29618 | biostudies-literature | 2001 Feb

REPOSITORIES: biostudies-literature