A novel approach for the identification of protein-protein interaction with integral membrane proteins.

ABSTRACT: Protein-protein interaction plays a major role in all biological processes. The currently available genetic methods such as the two-hybrid system and the protein recruitment system are relatively limited in their ability to identify interactions with integral membrane proteins. Here we describe the development of a reverse Ras recruitment system (reverse RRS), in which the bait used encodes a membrane protein. The bait is expressed in its natural environment, the membrane, whereas the protein partner (the prey) is fused to a cytoplasmic Ras mutant. Protein-protein interaction between the proteins encoded by the prey and the bait results in Ras membrane translocation and activation of a viability pathway in yeast. We devised the expression of the bait and prey proteins under the control of

SUBMITTER: Hubsman M 

PROVIDER: S-EPMC29625 | biostudies-literature | 2001 Feb

REPOSITORIES: biostudies-literature