Chemoenzymatic design of heparan sulfate oligosaccharides.

ABSTRACT: Heparan sulfate is a sulfated glycan that exhibits essential physiological functions. Interrogation of the specificity of heparan sulfate-mediated activities demands a library of structurally defined oligosaccharides. Chemical synthesis of large heparan sulfate oligosaccharides remains challenging. We report the synthesis of oligosaccharides with different sulfation patterns and sizes from a disaccharide building block using glycosyltransferases, heparan sulfate C(5)-epimerase, and sulfotransferases. This method offers a generic approach to prepare heparan sulfate oligosaccharides possessing predictable structures.

SUBMITTER: Liu R 

PROVIDER: S-EPMC2962522 | biostudies-literature | 2010 Oct

REPOSITORIES: biostudies-literature