The RAD51 and DMC1 homoeologous genes of bread wheat: cloning, molecular characterization and expression analysis.

ABSTRACT:

Background

Meiotic recombination in eukaryotes requires two homologues of the E. coli RecA proteins: Rad51 and Dmc1. Both proteins play important roles in the binding of single stranded DNA, homology search, strand invasion and strand exchange. Meiotic recombination has been well studied in Arabidopsis, rice, maize and the orthologues of RAD51 and DMC1 have been characterized. However genetic analysis of the RAD51 and DMC1 genes in bread wheat has been hampered due to the absence of complete sequence information and because of the existence of multiple copies of each gene in the hexaploid wheat genome.

Findings

In this study we have identified that TaRAD51 and TaDMC1 homoeologues are located on group 7 and group 5 chromosomes of hexaploid wheat, respectively. Comparative seq

SUBMITTER: Devisetty UK 

PROVIDER: S-EPMC2962619 | biostudies-literature | 2010 Sep

REPOSITORIES: biostudies-literature