Early embryonic gene expression profiling of zebrafish prion protein (Prp2) morphants.

ABSTRACT:

Background

The Prion protein (PRNP/Prp) plays a crucial role in transmissible spongiform encephalopathies (TSEs) like Creutzfeldt-Jakob disease (CJD), scrapie and mad cow disease. Notwithstanding the importance in human and animal disease, fundamental aspects of PRNP/Prp function and transmission remains unaccounted for.

Methodology/principal findings

The zebrafish (Danio rerio) genome contains three Prp encoding genes assigned prp1, prp2 and prp3. Currently, the second paralogue is believed to be the most similar to the mammalian PRNP gene in structure and function. Functional studies of the PRNP gene ortholog was addressed by prp2 morpholino (MO) knockdown experiments. Investigation of Prp2 depleted embryos revealed high mortality and apoptosis at 24 hours post fertilizati

SUBMITTER: Nourizadeh-Lillabadi R 

PROVIDER: S-EPMC2962645 | biostudies-literature | 2010 Oct

REPOSITORIES: biostudies-literature