Angiotensin receptor type 1 antagonists protect against neuronal injury induced by oxygen-glucose depletion.

ABSTRACT:

Background and purpose

Several clinical trials and in vivo animal experiments have suggested that blockade of angiotensin receptor type 1 (AT(1)) improves ischaemic outcomes. However, the mechanism(s) underlying these effects has not been elucidated. Here, we have investigated the protective effects of pretreatment with AT(1) receptor antagonists, losartan or telmisartan, against ischaemic insult to neurons in vitro.

Experimental approach

Primary rat neuron-astrocyte co-cultures and astrocyte-defined medium (ADM)-cultured pure astrocyte cultures were prepared. Ischaemic injury was modelled by oxygen-glucose depletion (OGD) and lactate dehydrogenase release after OGD was measured with or without AT(1) receptor antagonists or agonists (L162313), AT(2) receptor antagonist (PD12

SUBMITTER: Wu X 

PROVIDER: S-EPMC2962815 | biostudies-literature | 2010 Sep

REPOSITORIES: biostudies-literature