Expression of multidrug resistance associated protein 5 (MRP5) on cornea and its role in drug efflux.

ABSTRACT:

Purpose

The purpose of this manuscript is to investigate the presence of nucleoside/nucleotide efflux transporter in cornea and to evaluate the role in ocular drug efflux.

Methods

RT-PCR, immunoprecipitation followed by Western blot analysis and immunostaining were employed to establish molecular presence of multidrug resistance associated protein 5 (MRP5) on cornea. Corneal efflux by MRP5 was studied with bis(POM)-PMEA and acyclovir using rabbit and human corneal epithelial cells along with MRP5 over expressing cells (MDCKII-MRP5). Ex vivo studies using excised rabbit cornea and in vivo ocular microdialysis in male New Zealand white rabbits were used to further evaluate the role of MRP5 in conferring ocular drug resistance.

Results

RT-PCR confirms the expression of M

SUBMITTER: Karla PK 

PROVIDER: S-EPMC2963904 | biostudies-literature | 2009 Apr

REPOSITORIES: biostudies-literature