In vivo two-photon imaging reveals monocyte-dependent neutrophil extravasation during pulmonary inflammation.

ABSTRACT: Immune-mediated pulmonary diseases are a significant public health concern. Analysis of leukocyte behavior in the lung is essential for understanding cellular mechanisms that contribute to normal and diseased states. Here, we used two-photon imaging to study neutrophil extravasation from pulmonary vessels and subsequent interstitial migration. We found that the lungs contained a significant pool of tissue-resident neutrophils in the steady state. In response to inflammation produced by bacterial challenge or transplant-mediated, ischemia-reperfusion injury, neutrophils were rapidly recruited from the circulation and patrolled the interstitium and airspaces of the lung. Motile neutrophils often aggregated in dynamic clusters that formed and dispersed over tens of minutes. These clusters wer

SUBMITTER: Kreisel D 

PROVIDER: S-EPMC2964224 | biostudies-literature | 2010 Oct

REPOSITORIES: biostudies-literature