Azirine ligation: fast and selective protein conjugation via photoinduced azirine-alkene cycloaddition.

ABSTRACT: We report a new bioorthogonal ligation reaction between p-nitrodiphenylazirine and dimethyl fumarate. This photoinduced azirine-alkene cycloaddition provides a rapid (~2 min) and highly selective route to protein conjugation at neutral pH and room temperature in biological medium.

SUBMITTER: Lim RK 

PROVIDER: S-EPMC2964347 | biostudies-literature | 2010 Nov

REPOSITORIES: biostudies-literature