Characterization of exceptionally thermostable single-stranded DNA-binding proteins from Thermotoga maritima and Thermotoga neapolitana.

ABSTRACT:

Background

In recent years, there has been an increasing interest in SSBs because they find numerous applications in diverse molecular biology and analytical methods.

Results

We report the characterization of single-stranded DNA binding proteins (SSBs) from the thermophilic bacteria Thermotoga maritima (TmaSSB) and Thermotoga neapolitana (TneSSB). They are the smallest known bacterial SSB proteins, consisting of 141 and 142 amino acid residues with a calculated molecular mass of 16.30 and 16.58 kDa, respectively. The similarity between amino acid sequences of these proteins is very high: 90% identity and 95% similarity. Surprisingly, both TmaSSB and TneSSB possess a quite low sequence similarity to Escherichia coli SSB (36 and 35% identity, 55 and 56% similarity, respectivel

SUBMITTER: Olszewski M 

PROVIDER: S-EPMC2964679 | biostudies-literature | 2010 Oct

REPOSITORIES: biostudies-literature