Unique differentiation profile of mouse embryonic stem cells in rotary and stirred tank bioreactors.

ABSTRACT: Embryonic stem (ES)-cell-derived lineage-specific stem cells, for example, hematopoietic stem cells, could provide a potentially unlimited source for transplantable cells, especially for cell-based therapies. However, reproducible methods must be developed to maximize and scale-up ES cell differentiation to produce clinically relevant numbers of therapeutic cells. Bioreactor-based dynamic culture conditions are amenable to large-scale cell production, but few studies have evaluated how various bioreactor types and culture parameters influence ES cell differentiation, especially hematopoiesis. Our results indicate that cell seeding density and bioreactor speed significantly affect embryoid body formation and subsequent generation of hematopoietic stem and progenitor cells in both stirred ta

SUBMITTER: Fridley KM 

PROVIDER: S-EPMC2965195 | biostudies-literature | 2010 Nov

REPOSITORIES: biostudies-literature