Role of tumor necrosis factor-alpha in wound repair in human vocal fold fibroblasts.

ABSTRACT:

Objectives/hypothesis

Tumor necrosis factor-alpha (TNF-alpha) is an inflammatory cytokine and apoptotic molecule that appears to be a mediator in inflammation and fibrosis. The objective of this investigation was to examine the effects of TNF-alpha on 3D Carbylan-GSX in vitro cultured human vocal fold fibroblasts (hVFFs), to provide insight into the mechanism responsible for the improved vocal fold wound healing that has been previous reported with Carbylan-GSX treatment.

Study design

In vitro cell culture.

Methods

hVFF were cultured in 3D Carbylan-GSX and on polystyrene with different dosages of TNF-alpha (0, 0.1, 1, 10, and 100 ng/mL) with and without 10% fetal bovine serum (FBS). hVFF response to TNF-alpha was characterized by morphology, proliferation rates, and g

SUBMITTER: Chen X 

PROVIDER: S-EPMC2965638 | biostudies-literature | 2010 Sep

REPOSITORIES: biostudies-literature