Measurement and quality control issues in multiplex protein assays: a case study.

ABSTRACT:

Background

Multiplex arrays are increasingly used for measuring protein biomarkers. Advantages of this approach include specimen conservation, limited sample handling, and decreased time and cost, but the challenges of optimizing assay format for each protein, selecting common dilution factors, and establishing robust quality control algorithms are substantial. Here, we use measurements of 15 protein biomarkers from a large study to illustrate processing, analytic, and quality control issues with multiplexed immunoassays.

Methods

We contracted with ThermoScientific for duplicate measurements of 15 proteins in 2322 participants from a community-based cohort, a plasma control, and recombinant protein controls using 2 custom planar microarrays with 6 (panel A) or 9 (panel B) ca

SUBMITTER: Ellington AA 

PROVIDER: S-EPMC2965639 | biostudies-literature | 2009 Jun

REPOSITORIES: biostudies-literature