A new fluorescence-based method identifies protein phosphatases regulating lipid droplet metabolism.

ABSTRACT: In virtually every cell, neutral lipids are stored in cytoplasmic structures called lipid droplets (LDs) and also referred to as lipid bodies or lipid particles. We developed a rapid high-throughput assay based on the recovery of quenched BODIPY-fluorescence that allows to quantify lipid droplets. The method was validated by monitoring lipid droplet turnover during growth of a yeast culture and by screening a group of strains deleted in genes known to be involved in lipid metabolism. In both tests, the fluorimetric assay showed high sensitivity and good agreement with previously reported data using microscopy. We used this method for high-throughput identification of protein phosphatases involved in lipid droplet metabolism. From 65 yeast knockout strains encoding protein phosphatases and

SUBMITTER: Bozaquel-Morais BL 

PROVIDER: S-EPMC2965658 | biostudies-literature | 2010 Oct

REPOSITORIES: biostudies-literature