Wnt signalling in adenomas of familial adenomatous polyposis patients.

ABSTRACT:

Background

Epigenetic silencing of Wnt antagonists and expression changes in genes associated with Wnt response pathways occur in early sporadic colorectal tumourigenesis, indicating that tumour cells are more sensitive to Wnt growth factors and respond differently. In this study, we have investigated whether similar changes occur in key markers of the Wnt response pathways in the genetic form of the disease, familial adenomatous polyposis (FAP).

Methods

We investigated epigenetic and expression changes using pyrosequencing and real-time RT-PCR in samples from seven patients without neoplasia, and matched normal and tumour tissues from 22 sporadic adenoma and 14 FAP patients.

Results

We found that 17 out of 24 (71%) FAP adenomas were hypermethylated at sFRP1, compared

SUBMITTER: Caldwell GM 

PROVIDER: S-EPMC2966613 | biostudies-literature | 2010 Sep

REPOSITORIES: biostudies-literature