Precursor charge state prediction for electron transfer dissociation tandem mass spectra.

ABSTRACT: Electron-transfer dissociation (ETD) induces fragmentation along the peptide backbone by transferring an electron from a radical anion to a protonated peptide. In contrast with collision-induced dissociation, side chains and modifications such as phosphorylation are left intact through the ETD process. Because the precursor charge state is an important input to MS/MS sequence database search tools, the ability to accurately determine the precursor charge is helpful for the identification process. Furthermore, because ETD can be applied to large, highly charged peptides, the need for accurate precursor charge state determination is magnified. Otherwise, each spectrum must be searched repeatedly using a large range of possible precursor charge states. To address this problem, we have develop

SUBMITTER: Sharma V 

PROVIDER: S-EPMC2966942 | biostudies-literature | 2010 Oct

REPOSITORIES: biostudies-literature