The HUDSEN Atlas: a three-dimensional (3D) spatial framework for studying gene expression in the developing human brain.

ABSTRACT: We are developing a three-dimensional (3D) atlas of the human embryonic brain using anatomical landmarks and gene expression data to define major subdivisions through 12 stages of development [Carnegie Stages (CS) 12-23; approximately 26-56 days post conception (dpc)]. Virtual 3D anatomical models are generated from intact specimens using optical projection tomography (OPT). Using MAPAINT software, selected gene expression data, gathered using standard methods of in situ hybridization and immunohistochemistry, are mapped to a representative 3D model for each chosen Carnegie stage. In these models, anatomical domains, defined on the basis of morphological landmarks and comparative knowledge of expression patterns in vertebrates, are linked to a developmental neuroanatomic ontology. Human ge

SUBMITTER: Kerwin J 

PROVIDER: S-EPMC2967454 | biostudies-literature | 2010 Oct

REPOSITORIES: biostudies-literature