A protein-centric approach for the identification of folate enzymes from the malarial parasite, Plasmodium falciparum, using OFFGEL™ solution-based isoelectric focussing and mass spectrometry.

ABSTRACT:

Background

Plasmodium species are difficult to study using proteomic technology because they contain large amounts of haemoglobin-derived products (HDP), generated by parasite breakdown of host haemoglobin. HDP are known to interfere with isoelectric focussing, a cornerstone of fractionation strategies for the identification of proteins by mass spectrometry. In addition to the challenge presented by this material, as in most proteomes, there exists in this parasite a considerable dynamic range between proteins of high and low abundance. The enzymes of the folate pathway, a proven and widely used drug target, are included in the latter class.

Methods

This report describes a work-flow utilizing a parasite-specific extraction protocol that minimizes release of HDP into the lysa

SUBMITTER: O'Cualain RD 

PROVIDER: S-EPMC2967559 | biostudies-literature | 2010 Oct

REPOSITORIES: biostudies-literature