PCR candidate region mismatch scanning: adaptation to quantitative, high-throughput genotyping.

ABSTRACT: Linkage and association analyses were performed to identify loci affecting disease susceptibility by scoring previously characterized sequence variations such as microsatellites and single nucleotide polymorphisms. Lack of markers in regions of interest, as well as difficulty in adapting various methods to high-throughput settings, often limits the effectiveness of the analyses. We have adapted the Escherichia coli mismatch detection system, employing the factors MutS, MutL and MutH, for use in PCR-based, automated, high-throughput genotyping and mutation detection of genomic DNA. Optimal sensitivity and signal-to-noise ratios were obtained in a straightforward fashion because the detection reaction proved to be principally dependent upon monovalent cation concentration and MutL concentrat

SUBMITTER: Beaulieu M 

PROVIDER: S-EPMC29718 | biostudies-literature | 2001 Mar

REPOSITORIES: biostudies-literature