Intranasal vaccination with messenger RNA as a new approach in gene therapy: use against tuberculosis.

ABSTRACT:

Background

mRNAs are highly versatile, non-toxic molecules that are easy to produce and store, which can allow transient protein expression in all cell types. The safety aspects of mRNA-based treatments in gene therapy make this molecule one of the most promising active components of therapeutic or prophylactic methods. The use of mRNA as strategy for the stimulation of the immune system has been used mainly in current strategies for the cancer treatment but until now no one tested this molecule as vaccine for infectious disease.

Results

We produce messenger RNA of Hsp65 protein from Mycobacterium leprae and show that vaccination of mice with a single dose of 10 μg of naked mRNA-Hsp65 through intranasal route was able to induce protection against subsequent challenge with vi

SUBMITTER: Lorenzi JC 

PROVIDER: S-EPMC2972232 | biostudies-literature | 2010 Oct

REPOSITORIES: biostudies-literature