Disturbed expression of splicing factors in renal cancer affects alternative splicing of apoptosis regulators, oncogenes, and tumor suppressors.

ABSTRACT:

Background

Clear cell renal cell carcinoma (ccRCC) is the most common type of renal cancer. One of the processes disturbed in this cancer type is alternative splicing, although phenomena underlying these disturbances remain unknown. Alternative splicing consists of selective removal of introns and joining of residual exons of the primary transcript, to produce mRNA molecules of different sequence. Splicing aberrations may lead to tumoral transformation due to synthesis of impaired splice variants with oncogenic potential. In this paper we hypothesized that disturbed alternative splicing in ccRCC may result from improper expression of splicing factors, mediators of splicing reactions.

Methodology/principal findings

Using real-time PCR and Western-blot analysis we analyzed exp

SUBMITTER: Piekielko-Witkowska A 

PROVIDER: S-EPMC2972751 | biostudies-literature | 2010 Oct

REPOSITORIES: biostudies-literature