Remodeling of protein and mRNA expression in Leishmania mexicana induced by deletion of glucose transporter genes.

ABSTRACT: Glucose is a major nutrient in the insect vector stage of Leishmania parasites. Glucose transporter null mutants of Leishmania mexicana exhibit profound phenotypic changes in both insect stage promastigotes and mammalian host stage amastigotes that reside within phagolysosomes of host macrophages. Some of these phenotypic changes could be either mediated or attenuated by changes in gene expression that accompany deletion of the glucose transporter genes. To search for changes in protein expression, the profile of proteins detected on two-dimensional gels was compared for wild type and glucose transporter null mutant promastigotes. A total of 50 spots whose intensities changed significantly and consistently in multiple experiments were detected, suggesting that a cohort of proteins is alter

SUBMITTER: Feng X 

PROVIDER: S-EPMC2974008 | biostudies-literature | 2011 Jan

REPOSITORIES: biostudies-literature