Novel approach to gene expression profiling in Sezary syndrome.

ABSTRACT:

Background

Microarray hybridization studies in Sézary syndrome (SS) have compared T lymphocytes from patients with cutaneous T-cell lymphoma with those of normal controls; a major limitation of this design is that significant inherent genetic variability of lymphocyte populations between individuals may produce differences in gene expression unrelated to disease state.

Objective

The objective of this study was to minimize the heterogeneity of information derived from whole-genome expression analysis and to identify specific genetic differences between highly purified malignant and nonmalignant (control) T cells from the same patient with SS.

Methods

Peripheral blood mononuclear cells were obtained from a patient with SS, stained with anti-T-cell receptor Vb (TCR-Vb) a

SUBMITTER: Pomerantz RG 

PROVIDER: S-EPMC2974048 | biostudies-literature | 2010 Nov

REPOSITORIES: biostudies-literature