Optimising experimental design for high-throughput phenotyping in mice: a case study.

ABSTRACT: To further the functional annotation of the mammalian genome, the Sanger Mouse Genetics Programme aims to generate and characterise knockout mice in a high-throughput manner. Annually, approximately 200 lines of knockout mice will be characterised using a standardised battery of phenotyping tests covering key disease indications ranging from obesity to sensory acuity. From these findings secondary centres will select putative mutants of interest for more in-depth, confirmatory experiments. Optimising experimental design and data analysis is essential to maximise output using the resources with greatest efficiency, thereby attaining our biological objective of understanding the role of genes in normal development and disease. This study uses the example of the noninvasive blood pressure tes

SUBMITTER: Karp NA 

PROVIDER: S-EPMC2974211 | biostudies-literature | 2010 Oct

REPOSITORIES: biostudies-literature