RH genotyping in a sickle cell disease patient contributing to hematopoietic stem cell transplantation donor selection and management.

ABSTRACT: African individuals harbor molecular RH variants, which permit alloantibody formation to high-prevalence Rh antigens after transfusions. Genotyping identifies such RH variants, which are often missed by serologic blood group typing. Comprehensive molecular blood group analysis using 3 genotyping platforms, nucleotide sequencing, and serologic evaluation was performed on a 7-year-old African male with sickle cell disease who developed an "e-like" antibody shortly after initiating monthly red blood cell (RBC) transfusions for silent stroke. Genotyping of the RH variant predicted a severe shortage of compatible RBCs for long-term transfusion support, which contributed to the decision for hematopoetic stem cell transplantation. RH genotyping confirmed the RH variant in the human leukocyte anti

SUBMITTER: Fasano RM 

PROVIDER: S-EPMC2974591 | biostudies-literature | 2010 Oct

REPOSITORIES: biostudies-literature