Hematopoiesis in 3 dimensions: human and murine bone marrow architecture visualized by confocal microscopy.

ABSTRACT: In many animals, blood cell production occurs in the bone marrow. Hematopoiesis is complex, requiring self-renewing and pluripotent stem cells, differentiated progenitor and precursor cells, and supportive stroma, adipose tissue, vascular structures, and extracellular matrix. Although imaging is a vital tool in hematology research, the 3-dimensional architecture of the bone marrow tissue in situ remains largely uncharacterized. The major hindrance to imaging the intact marrow is the surrounding bone structures are almost impossible to cut/image through. We have overcome these obstacles and describe a method whereby whole-mounts of bone marrow tissue were immunostained and imaged in 3 dimensions by confocal fluorescence and reflection microscopy. We have successfully mapped by multicolor im

SUBMITTER: Takaku T 

PROVIDER: S-EPMC2974595 | biostudies-literature | 2010 Oct

REPOSITORIES: biostudies-literature