Proteomic analysis of the dysferlin protein complex unveils its importance for sarcolemmal maintenance and integrity.

ABSTRACT: Dysferlin is critical for repair of muscle membranes after damage. Mutations in dysferlin lead to a progressive muscular dystrophy. Recent studies suggest additional roles for dysferlin. We set out to study dysferlin's protein-protein interactions to obtain comprehensive knowledge of dysferlin functionalities in a myogenic context. We developed a robust and reproducible method to isolate dysferlin protein complexes from cells and tissue. We analyzed the composition of these complexes in cultured myoblasts, myotubes and skeletal muscle tissue by mass spectrometry and subsequently inferred potential protein functions through bioinformatics analyses. Our data confirm previously reported interactions and support a function for dysferlin as a vesicle trafficking protein. In addition novel poten

SUBMITTER: de Morree A 

PROVIDER: S-EPMC2974636 | biostudies-literature | 2010 Nov

REPOSITORIES: biostudies-literature