Butyrate suppresses expression of neuropilin I in colorectal cell lines through inhibition of Sp1 transactivation.

ABSTRACT:

Background

Neuropilin is a transmembrane receptor for vascular endothelial growth factor (VEGF) and is expressed in normal endothelial cells and upregulated in cancer cells. Neuropilin-1 (NRP-1) has been shown to promote tumour cell migration and survival in colon cancer in response to VEGF binding. The expression profiles of neuropilins, associated co-receptors and known ligands have been mapped in three colorectal cell lines: Caco-2, HCT116 & HT29. We have previously shown that butyrate, a naturally occurring histone deacetylase inhibitor (HDACi) produced by fermentation of fibre in the colon, causes apoptosis of colon cancer cell lines.

Results

Here we demonstrate that butyrate down-regulates NRP-1 and VEGF at the mRNA and protein level in colorectal cancer cell lines. NR

SUBMITTER: Yu DC 

PROVIDER: S-EPMC2974727 | biostudies-literature | 2010 Oct

REPOSITORIES: biostudies-literature