Identification of differentially expressed genes using an annealing control primer system in stage III serous ovarian carcinoma.

ABSTRACT:

Background

Most patients with ovarian cancer are diagnosed with advanced stage disease (i.e., stage III-IV), which is associated with a poor prognosis. Differentially expressed genes (DEGs) in stage III serous ovarian carcinoma compared to normal tissue were screened by a new differential display method, the annealing control primer (ACP) system. The potential targets for markers that could be used for diagnosis and prognosis, for stage III serous ovarian cancer, were found by cluster and survival analysis.

Methods

The ACP-based reverse transcriptase polymerase chain reaction (RT PCR) technique was used to identify DEGs in patients with stage III serous ovarian carcinoma. The DEGs identified by the ACP system were confirmed by quantitative real-time PCR. Cluster analysis was

SUBMITTER: Kim YS 

PROVIDER: S-EPMC2974737 | biostudies-literature | 2010 Oct

REPOSITORIES: biostudies-literature