Serum autoantibody profiling using a natural glycoprotein microarray for the prognosis of early melanoma.

ABSTRACT: The poor prognosis of melanoma and the high cost of lymph node biopsy for melanoma patients have led to an urgent need for the discovery of convenient and accurate prognostic indicators. Here, we have developed a natural glycoprotein microarray to discover serum autoantibodies to distinguish between patients with node negative melanoma and node positive melanoma. Dual-lectin affinity chromatography was used to extract glycoproteins from a melanoma cell line. Liquid-based reverse phase separation and microarray platforms were then applied to separate and spot these natural proteins on nitrocellulose slides. The serum autoantibodies were investigated by exposing these proteins to sera from 43 patients that have already been diagnosed to have different stages of early melanoma. The combinatio

SUBMITTER: Liu Y 

PROVIDER: S-EPMC2974814 | biostudies-literature | 2010 Nov

REPOSITORIES: biostudies-literature