Biochemical characterization of the transcriptional regulator BzdR from Azoarcus sp. CIB.

ABSTRACT: The BzdR transcriptional regulator that controls the P(N) promoter responsible for the anaerobic catabolism of benzoate in Azoarcus sp. CIB constitutes the prototype of a new subfamily of transcriptional regulators. Here, we provide some insights about the functional-structural relationships of the BzdR protein. Analytical ultracentrifugation studies revealed that BzdR is homodimeric in solution. An electron microscopy three-dimensional reconstruction of the BzdR dimer has been obtained, and the predicted structures of the respective N- and C-terminal domains of each BzdR monomer could be fitted into such a reconstruction. Gel retardation and ultracentrifugation experiments have shown that the binding of BzdR to its cognate promoter is cooperative. Different biochemical approaches revealed

SUBMITTER: Durante-Rodriguez G 

PROVIDER: S-EPMC2975194 | biostudies-literature | 2010 Nov

REPOSITORIES: biostudies-literature